SP SEPLITE® FF Chromatographic Media

Agarose matrix, SP group, fast flow, Equivalent to SP Sepharose FF

• Column selection: Theoretically, as long as the column is long enough, the ideal resolution can be obtained, but since the column flow rate is related to the pressure gradient, the column length increases to slow the flow rate, the peak becomes wider, and the resolution Decrease; the diameter of the column increases, causing an increase in the non-uniformity of the liquid flow and a significant decrease in resolution.
• The pH and ionic strength of the elution buffer must be strictly controlled during the purification process. Column chromatography should be performed after the sample and the SP chromatography medium must be thoroughly equilibrated with the elution buffer.
• The column bed must be flat, without grooves and air bubbles, otherwise it should be repacked.
• The flow rate should be strictly controlled during the elution process, and not too fast.
• The volume of the sample should be small and the concentration should not be too high.
• During the loading and the entire elution process, the cylinder surface is prevented from drying out.

Agarose Chromatography Resin