SP SEPLITE® FF Chromatographic Media

SP SEPLITE® FF Chromatographic Media

Catalog Number:
CM01110683SUN
Mfr. No.:
SPSeplifeFF
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    • Overview

      • SP seplife® FF chromatography resin is a new highly cross-linked agarose chromatography resin independently developed by Sunresin. It is a kind of sulfonic acid-based propyl bonded on agarose gel filtration chromatography resin. Strong cation exchange chromatography resin with high flow rate, low back pressure, high dynamic loading, good chemical stability and mechanical properties, low non-specific adsorption, high recovery rate, convenient scale-up, shortening production time and improving Productivity. It is widely used in ion exchange separation of proteins, nucleic acids and peptides downstream of biopharmaceuticals and bioengineering.

        Please contact us at for specific academic pricing.

    • Properties
      • Categories
        Agarose matrix, SP group, fast flow, Equivalent to SP Sepharose FF
        Note
        • Column selection: Theoretically, as long as the column is long enough, the ideal resolution can be obtained, but since the column flow rate is related to the pressure gradient, the column length increases to slow the flow rate, the peak becomes wider, and the resolution Decrease; the diameter of the column increases, causing an increase in the non-uniformity of the liquid flow and a significant decrease in resolution.
        • The pH and ionic strength of the elution buffer must be strictly controlled during the purification process. Column chromatography should be performed after the sample and the SP chromatography medium must be thoroughly equilibrated with the elution buffer.
        • The column bed must be flat, without grooves and air bubbles, otherwise it should be repacked.
        • The flow rate should be strictly controlled during the elution process, and not too fast.
        • The volume of the sample should be small and the concentration should not be too high.
        • During the loading and the entire elution process, the cylinder surface is prevented from drying out.

        * For Research Use Only.

    • Applications
      • Application Description
        Agarose Chromatography Resin

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