AMPK Signaling Phospho Antibody Array

AMPK Phospho Antibody Array features 174 highly specific antibodies important to the AMPK (AMP-activated protein kinase) signaling pathway. The array is designed for protein phosphorylation profiling protein in cell and tissue samples from human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

AMPK Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. The array is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine phosphorylation and serine/threonine phosphorylation at specific sites.

Key Features:
· Site-specific phosphorylation profiling and screening with 174 antibodies
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Fluorescent detection

Specifications:
· Product Size: 2 array slides per package for two samples (untreated vs. treated)
· Featured Antibodies: 174 site-specific and phospho-specific antibodies
· Reactivity: Human: 100% | Mouse: 89% | Rat: 77%
· Suitable Sample Type: Cell lysate | Tissue lysate
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: Positive controls: beta-actin | GAPDH | Negative controls
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
4E-BP1 (Ser65), 4E-BP1 (Thr36), 4E-BP1 (Thr45), 4E-BP1 (Thr70), ACC1 (Ser79), ACC1 (Ser80), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT1/2/3, AKT1S1 (Thr246), AKT2 (Ser474), AMPK1 (Thr174), AMPK1/AMPK2 (Ser485/491), AMPKbeta1 (Ser182), CaMK1-alpha (Thr177), CaMK1-beta, CaMK2 (Thr305), CaMK2 (Thr286), CaMK2-b/g/d (Thr287), CaMK4 (Thr196/200), CAMK5, Cyclin A1, Cyclin B1 (Ser126), Cyclin B1 (Ser147), EEF2 (Thr56), eEF2K (Ser366), eNOS (Ser1177), eNOS (Ser615), eNOS (Thr495), Fas ligand, HMG14, HMG20B, HMGB1, HMGB2, HNF4a (Ser304), HNF4a/g, HSF1 (Ser303), HSL (Ser552/563), HSL (Ser554), LKB1 (Ser428), LKB1 (Thr189), MEF2A (Ser408), MEF2A (Thr312), MEF2A (Thr319), MEF2B, MEF2C (Ser396), MEF2D (Ser444), mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), p21Cip1 (Thr145), p53 (Ser6), p53 (Ser9), p53 (Ser15), p53 (Ser20), p53 (Ser33), p53 (Ser37), p53 (Ser46), p53 (Ser315), p53 (Ser366), p53 (Ser378), p53 (Ser392), p53 (Thr18), p53 (Thr81), p70S6K (Ser371), p70S6K (Ser411), p70S6K (Ser418), p70S6K (Ser424), p70S6K (Thr229), p70S6K (Thr389), p70S6k (Thr421), p70S6K-beta (Ser423), PFKFB2 (Ser483), PI3K-p85-alpha (Tyr607), PI3K-p85-a/g (Tyr467/199), PKA CAT (Thr197), PKA-R2B (Ser113), PLC-beta (Ser1105), PLC-beta3 (Ser537), RapGEF1 (Tyr504), SREBP-1 (Ser439), Tuberin (Ser939), Tuberin (Thr1462)

Assay Outline:
The ELISA based AMPK Pathway Phospho Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.