Name: Sheep anti Rat alpha foetoprotein, conjugated with FITC
Price: 570.00 USD

- Overview
  - FITC-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the activity of the conjugate. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
    
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    Background
    
    The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against amniotic fluid, rat hepatoma sera and purified alpha-foetoprotein an single characteristic precipitin line is obtained. No reaction is obtained with rat sera of different strains. To identify and measure alpha foetoprotein at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates and to identify and measure alpha-foetoprotein in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
- Properties
  - Host
    
    sheep
    
    Antibody Type
    
    polyclonal
    
    Formulation
    
    FITC-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the activity of the conjugate.
    
    Storage
    
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
    
    Note
    
    This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
    
    * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
- Applications
  - Application Description
    
    ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence.

Datasheet

Sheep anti Rat alpha foetoprotein, conjugated with FITC

Sheep anti Rat alpha foetoprotein, conjugated with FITC

Background

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