Name: Sheep anti Mouse Ig lambda light chains (free and bound), conjugated with Biotin
Price: 568.00 USD

- Overview
  - Biotin-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda chain. A reaction is obtained with intact polyclonal or monoclonal immunoglobulins of the lambda type, as well as free lambda light chains. To identify light chain type in immunocytochemical and immunohistochemical staining of immunoglobulins and to demonstrate circulating monoclonal immunoglobulins and Bence Jones proteins of the lambda type in serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
- Properties
  - Host
    
    sheep
    
    Antibody Type
    
    polyclonal
    
    Formulation
    
    Biotin-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
    
    Storage
    
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
    
    Note
    
    This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
    
    * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
- Applications
  - Application Description
    
    ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting.

Datasheet

Sheep anti Mouse Ig lambda light chains (free and bound), conjugated with Biotin

Sheep anti Mouse Ig lambda light chains (free and bound), conjugated with Biotin

Background

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