Name: Sheep anti Human salivary amylase
Price: 420.00 USD

- Overview
  - Delipidated, heat inactivated, lyophilized, stable antiserum. No preservative added Total protein and IgG concentration are comparable to those of pooled normal sheep serum. No foreign proteins added Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
    
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    Background
    
    The reactivity of the antiserum is restricted to salivary amylase. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human saliva a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. No reaction is obtained with human plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of salivary amylase or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibodybinding techniques if proper controls are included.
- Properties
  - Host
    
    sheep
    
    Antibody Type
    
    polyclonal
    
    Formulation
    
    Delipidated, heat inactivated, lyophilized, stable antiserum. No preservative added Total protein and IgG concentration are comparable to those of pooled normal sheep serum. No foreign proteins added
    
    Storage
    
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
    
    Note
    
    This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
    
    * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
- Applications
  - Application
    
    Immunoelectrophoresis; Immunodiffusion
    
    Application Description
    
    Precipitation assays. In immunoelectrophoresis in agarose-plates use 2 μl human saliva or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Datasheet

Sheep anti Human salivary amylase

Sheep anti Human salivary amylase

Background

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