Name: Sheep anti Human inter alpha trypsin inhibitor
Price: 340.00 USD

- Overview
  - Delipidated, heat inactivated, lyophilized, stable IgG fraction in ½ diluted normal serum. No preservative added. IgG fraction 13 mg/ml in normal sheep serum diluted 1/2 with PBS. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
    
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    Background
    
    The reactivity of the antiserum is restricted to inter alpha trypsin inhibitor. In immunoelectro- phoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. In precipitating techniques as immunoelectrophoresis and single and double radial immuno- diffusion (Mancini, Ouchterlony) to identify the presence of inter alpha trypsin inhibitor or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
- Properties
  - Host
    
    sheep
    
    Antibody Type
    
    polyclonal
    
    Formulation
    
    Delipidated, heat inactivated, lyophilized, stable IgG fraction in _ diluted normal serum. No preservative added. IgG fraction 13 mg/ml in normal sheep serum diluted 1/2 with PBS. No foreign proteins added.
    
    Storage
    
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
    
    Note
    
    This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
    
    * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
- Applications
  - Application
    
    Immunoelectrophoresis; Immunodiffusion
    
    Application Description
    
    Precipitation assays. In immunoelectrophoresis in agarose-plates use 2 μl human plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl anti- serum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion (Laurell) use a concentration of 1% of the antiserum in the gel.

Datasheet

Sheep anti Human inter alpha trypsin inhibitor

Sheep anti Human inter alpha trypsin inhibitor

Background

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