SDS PAGE Sample Buffer (2X) (Reducing) (Laemmli Buffer 2X)

A ready-to-use 2X solution with beta-mercaptoethanol as a reducing agent. Used in SDS-PAGE for loading of conventional proteins. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. Role of 2X SDS PAGE Sample buffer :1 .Tris-HCl is a buffer and it’s pH plays an essential role in preserving peptide bonds from breaking apart.2. Sodium dodecyl sulphate is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge.3. The high density of glycerol ensures the sample moves down into the well.4. Beta-mercaptoethanol, along with SDS, ensure the bands are individual polypeptide instead of molecular complexes.5. The dye visually indicates the location (tracking) of the sample in the gel.

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