Sample Release Reagent

Sample Release Reagent provide a simple, safe and effective method to release and preserve nucleic acid from clinical samples containing pathogens. This reagent releases total nucleic acids (DNA and RNA) from cells by lysing the cells with surfactants in an alkaline environment. This reagent can quickly release nucleic acid from freshly collected clinical samples, and protect nucleic acid from degradation.

Product Principle
Sample Release Reagent provide a simple, safe and effective method to release and preserve nucleic acid from clinical samples containing pathogens. This reagent releases total nucleic acids (DNA and RNA) from cells by lysing the cells with surfactants in an alkaline environment. This reagent can quickly release nucleic acid from freshly collected clinical samples, and protect nucleic acid from degradation.

Clinical samples, including vaginal swab, cervical swab, oral swab, pharyngeal swab, nasal swab, peripheral blood, urine, bacteria, viruses, fungi, mycoplasma, chlamydia, and cultures of the above pathogens, preprocessed by Sample Release Reagent can be directly used for molecular detection, such as PCR, RT-PCR, digital PCR and amplicon quenching etc., without heating or other nucleic acid extraction operations of samples. Fungal samples added to this product can have their cell walls quickly lysed by shaking treatment (shaking treatment is also applicable to bacterial species whose cell walls are difficult to be lysed).

Main component
It is mainly composed of NaOH, yeast total RNA, TWEEN-20, betaine, grinding beads and water.

Method of Application
· Swab samples
Clean hands before sampling, tear off the outer packaging of the swab, and take the swab out. Be careful not to touch the swab head.
Use a swab to sample the corresponding part according to different sampling requirements.
After sampling, quickly put the swab into the sampling tube containing sample release reagent to avoid contact with other parts.
Vigorously shake the tube by a vortex or other instrument for 20s, followed by a 10s pause and repeat 4 times (optional step), the nucleic acid will release into the agent.
· Peripheral blood
Peripheral blood must be added to this reagent in a fixed volume ratio (blood: reagent = 1:49)
Vigorously shake the tube by a vortex or other instrument for 20s, followed by a 10s pause and repeat 4 times (optional step), the nucleic acid will release into the agent.
· Urine
Take out not less than 3ml of urine, centrifuge at 4000rpm/min for 3 minutes and discard the supernatant.
Mix the urine sediment with this reagent thoroughly.
Vigorously shake the tube by a vortex or other instrument for 20s, followed by a 10s pause and repeat 4 times (optional step), the nucleic acid will release into the agent.
· Pathogen colonies
Pick up the colony and place it in the tube with reagent.
Vigorously shake the tube by a vortex or other instrument for 20s, followed by a 10s pause and repeat 4 times, the nucleic acid will release into the agent.

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