S-adenosylmethionine (SAM) FCM kit with Alexa Fluor® 647

S-adenosylmethionine, a sulfur-containing molecule, is a biologically very active metabolite and its important roles in methylation as a key methyl donor, transsulfuration and aminopropylation have been well understood. SAM is an intrinsically unstable molecule with molecular weight of 398.44, and its optical density maximum of 258-260 nm is not a distinguished absorption, accurate and timely determination of its concentration in various biological fluids and tissues has always been a challenging task. Therefore, finding a way that can quickly and specifically determine the amount of SAM becomes essential. With the advent of specific anti-SAM monoclonal antibodies, measure SAM from living cells directly, quickly and sensitively becomes possible with flow cytometry. Alexa Fluor® 647conjugated mouse anti-SAM antibody has been used (Refer to Cat# MAF00201) after cells are fixed and permeabilized. In this kit, both cytoplasm and nucleus fixation/permeabilization buffers have been include. With nucleus fixation/permeabilization system, all targets from cytoplasm and nucleus will be detected, whereas with cytoplasm fixation/permeabilization buffer, only cytoplasmic portion of the target is detected.

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