S-adenosylhomocysteine (SAH) FCM kit with Alexa Fluor® 488

S-adenosylhomocysteine, a sulfur-containing molecule, is an important molecule in methylation and transsulfuration. SAH does not have a distinguished absorption at 258-260 nm, accurate and timely determination of its concentration in various biological fluids and tissues has always been a challenging task. Therefore, finding a way that can quickly and specifically determine the amount of SAH becomes essential. With the advent of specific anti-SAH monoclonal antibodies, measure SAH from living cells directly, quickly and sensitively becomes possible with flow cytometry. Alexa Fluor® 488-conjugated mouse anti-SAH antibody has been used (Refer to Cat# MAF00301) after cells are fixed and permeabilized. In this kit, both cytoplasm and nucleus fixation/permeabilization buffers have been include. With nucleus fixation/permeabilization system, all targets from cytoplasm and nucleus will be detected, whereas with cytoplasm fixation/permeab小zation buffer, only cytoplasmic portion of the target is detected.

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