rLys-C Protease, 25u, Mass Spectrometry Grade

rLys-C Protease, 25u, Mass Spectrometry Grade

Catalog Number:
E001554775LIF
Mfr. No.:
HLS LYS001C
Price:
$306
  • Size:
    20 μg
    Quantity:
    Add to Cart:
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      • Overview

        • Physical Form: Mass spectrometry-grade rLys-C protease is provided as a lyophilized powder containing 0.5 mg trehalose per vial.
          Reconstitution Solution: rLys-C protease reconstitution buffer — recommended to use 1 mM HCl or 50 mM acetic acid.
          pH Range: rLys-C exhibits optimal activity at pH 8–9.
          Application: rLys-C protease specifically hydrolyzes denatured proteins at lysine (K) residues that are less susceptible to digestion. Co-digestion with trypsin is recommended.

          Recommended SOP:
          General solution digestion method
          Use a mixture of 100 µg trypsin and 20 µg rLys-C at a protein-to-enzyme ratio of 25:1 (w/w), incubate at 37°C for 13–16 hours overnight.

          Cleavage Sites: K

          Please contact us at for specific academic pricing.

          More Details

          Mass Spectrometry Grade Enzymes for Proteomic Digestion and Sample Preparation
      • Properties
        • Storage
          Lyophilized powder should be stored at –20°C. After reconstitution, the enzyme solution should also be stored at –20°C. Refer to the product label for the specific shelf life.
          Note
          The urea concentration should preferably be below 1 M. Use 50 mM Tris-HCl buffer (pH 8).
          Molecular Weight
          27kDa.

          * For Research Use Only.

      • Applications
        • Application Description
          Mass Spectrometry-grade rLys-C Protease specifically hydrolyzes peptide bonds at the C-terminus of lysine (K) residues. It can be used to enhance digestion of proteins that are difficult to cleave and is recommended to be performed in 6–8 M urea solution.

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