Rifampicin

RFP, Rifampin, 3-(((4-Methyl-1-piperazinyl)imino)methyl)rifamycin SV, NIH 10782, NSC 113926

13292-46-1

C43H58N4O12

822.94

Brownish-red crystalline powder

Source: Semi-synthetic: Amycolatopsis Rifamycinica
PH: 4.5-6.5
Assay: (On Dried Basis): 97.0-102.0%
Residue On Ignition: ≤ 0.1%
Heavy Metals: ≤20 ppm
Loss on Drying: ≤1.0%

Protect from light at -20°C.

Spectrum: Rifampicin is a broad-spectrum antibiotic with a wide range of activity including:Gram-positive aerobic bacteria, particularly Staphylococcus spp and Rhodococcus equi Brucella and some other fastidious organisms are susceptible but Gram-negative bacteria more generally are resistant Gram-positive and Gram-negative anaerobic bacteria are inhibited at low concentrations, including Bacteroides fragilis Chlamydophila and Rickettsia are susceptible Mycobacterium tuberculosis: activity is high against this organism but most other mycobacteria are resistant Some protozoa Some fungi and poxviruses
Microbiology Applications: Rifampicin is commonly used in bacterial recombinant protein expression to inhibit bacterial RNA polymerase activity and synthesis of host bacterial proteins. Rifampicin can also be used as a selective agent to isolate Campylobacter jejuni. Rose et al. used rifampicin from TOKU-E in methacrylate-based copolymer films and studied its effects on biofilm formation: "Prevention of Biofilm Formation by Methacrylate-Based Copolymer Films Loaded With Rifampin, Clarithromycin, Doxycycline Aone or in Combination." Media Supplements Rifampicin can be used as a selective agent in several types of isolation media: Campylobacter Agar - Campylobacter Selective Supplement (Preston) Campylobacter Agar Base - Modified Preston Campylobacter Selective Supplement

Plant Biology Applications: Rifampicin has been tested in Jerusalem artichoke tuber explants by adding 10 to 50 µg/ml to the tissue culture medium. At 50 µg/ml no bacterial infection was detectable, without affecting cell division rates, cytodifferentiation and DNA synthesis. As a result Rifampicin was used as antibacterial in the following experiments of this university department (Philips, 1981).Rifampicin is a selective inhibitor of chloroplast RNA polymerase and can be used to study chloroplast-level DNA transcription in plants.

Eukaryotic Cell Culture Applications: Rifampicin has been shown to have immunosuppressive effects in mice. There are no immunosuppressive effects in humans when rifampicin is given in doses at or below clinically recommended levels. Rifampicin has been shown to inhibit α-synuclein fibrillation and disaggregate fibrils in a concentration-dependent manner. Rifampicin can activate pregnane X receptor (PXR), which affects cytochrome P450, and the activity of glucuronosyltransferases and P-glycoprotein. Rifampicin has been shown to enhance CYP2C-mediated metabolism, affect compounds that are transported by P-glycoprotein and metabolized by CYP3A4.