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Overview
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Retro-CAG-eGFP-RG was packaged in HEK293T cells with Ca3(PO4)2 transfection of 3 plasmids of pRetro-CAGGFP, pRetro-RC, and pCMV-RG. The pRetro-CAG-GFP was constructed by using the components of Moloney Murine Leukemia virus (MoMLV), a combination of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter (CAG), and enhanced green fluorescent protein (eGFP) expression cassette.
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