rEndo S2 Glycosidase, Mass Spectra Grade

rEndo S2 Glycosidase is derived from Streptococcus pyogenes and is an IgG-specific endoglycosidase expressed in E. coli. This enzyme specifically hydrolyzes N-glycans following GlcNAc on the Fc region of native IgG, leaving GlcNAc intact or retaining the attached fucose residue. It can cleave Fc N-glycans from all human IgG subclasses and many IgGs from other species. Applications include studying antibody afucosylation levels, deglycosylated antibody immune affinity properties, ADCC effects lacking core fucose on N-glycans, site-specific conjugation of ADC drugs on Fc core GlcNAcs, and assessing IgG fucosylation levels in serum.

Physical Form: Lyophilized powder in 10 mM phosphate buffer (PB, pH 7.4 at 25°C) with 150 mM NaCl
Content: 20 µg
Shelf Life: 24 months when stored at –20°C
Reconstitution: Dissolve in 80 µL of water before use
Optimal pH Range: rEndo S2 glycosidase exhibits maximal activity at pH 7.4

Digestion Protocol:
· Recommended Digestion Buffer: 20 mM PB or Tris buffer, pH 7–8
· Sample Requirements: IgG protein samples at a protein-to-enzyme ratio of 200:1 (w/w), with a protein concentration of 1 µg/µL
· Incubation Conditions: 37°C dry bath for ≥30 minutes

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