Name: Recombinant Yeast Thioredoxin
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- Overview
  - Thioredoxin Yeast Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kDa. Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
    
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- Properties
  - Protein Name
    
    Thioredoxin-1, Thioredoxin I, TR-I, Thioredoxin-2, TRX1, TRX2, YLR043C.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 95.0% as determined by SDS-PAGE.
    
    Formulation
    
    Each mg of protein contains 20mM phosphate buffer pH 7.4.
    
    Storage
    
    TRX although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    It is recommended to reconstitute the lyophilized TRX in sterile 18MΩ-cm H2O. TRX activity is assayed by measuring the change in absorbance at 650 nm at 25°C using 0.13µM bovine insulin containing 0.33mM DTT (pH 6.5).The specific activity was found to be 5.8units/mg.

Recombinant Yeast Thioredoxin

Recombinant Yeast Thioredoxin

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