Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar

Catalog Number:
P001412508ABE
Mfr. No.:
Abe32-8732
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      • Overview
        • MW : 20.6kD. Recombinant S. hygroscopicus PPT N-acetyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed. Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.

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      • Properties
        • Source
          E. coli
          Type
          Recombinant Proteins
          Formulation
          Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
          Storage
          Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
          Endotoxin
          Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

          More Information

          UniProt

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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