Recombinant Klebsiella pneumoniae Kanamycin kinase type II/NEO

Recombinant Klebsiella pneumoniae Kanamycin kinase type II/NEO

Catalog Number:
P001412509ABE
Mfr. No.:
Abe32-8733
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      • Overview
        • MW : 29kD. Recombinant Klebsiella pneumoniae Aminoglycoside 3-phosphotransferase is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed. Aminoglycoside 3-phosphotransferase (APH(3)), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3) is primarily found in certain species of gram-positive bacteria.

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      • Properties
        • Source
          E. coli
          Type
          Recombinant Proteins
          Formulation
          Supplied as a 0.2 µm filtered solution of PBS, pH 7.4, 20% Glycerol.
          Storage
          Store at -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
          Endotoxin
          Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

          More Information

          UniProt

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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