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Overview
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MW : 29kD. Recombinant Klebsiella pneumoniae Aminoglycoside 3-phosphotransferase is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed. Aminoglycoside 3-phosphotransferase (APH(3)), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3) is primarily found in certain species of gram-positive bacteria.
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Overview