Name: Recombinant Protein G, His-tagged
Price: 502.00 USD

- Overview
  - The Protein G is a recombinant protein, expressed in E.coli cells, without the albumin binding site. The protein consists of 223 amino acids and a N-terminal His6-tag and one cysteine residue. Protein G was found to be a powerful reagent for the detection of IgG, and consequently the antigen against which these antibodies are directed. It was used in Western blot analyses to detect various antigen-antibody complexes on nitrocellulose membranes. Moreover, protein G is widely used as a ligand coupled to resins in affinity chromatography for antibody purification.
    
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- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    More than 90% of total protein
    
    Formulation
    
    The protein is solubilized in 20 mM Na-Phosphate pH 7.2, 150 mM NaCl.
    
    Storage
    
    Protein G is stable until the expiry date given on the label when stored at -70 °C. The protein can be kept at -20 °C for several weeks and on ice for several days. Repeated freezing and thawing should be avoided.
    
    Molecular Weight
    
    24.6 kDa
    
    * For Research Use Only
- Applications
  - Application Description
    
    Protein G was found to be a powerful reagent for the detection of IgG, and consequently the antigen against which these antibodies are directed. It was used in Western blot analyses to detect various antigen-antibody complexes on nitrocellulose membranes. Moreover, protein G is widely used as a ligand coupled to resins in affinity chromatography for antibody purification.

Datasheet

Recombinant Protein G, His-tagged

Recombinant Protein G, His-tagged

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