Name: Recombinant Protein A, His-tagged
Price: 502.00 USD

- Overview
  - Protein A is a recombinant protein, expressed in E.coli cells. The protein consists of 257 amino acids and a N-terminal His6-tag and one cysteine residue. Protein A was found to be a powerful reagent for the detection of IgG, and consequently the antigen against which these antibodies are directed. Protein A is widely used as a ligand coupled to resins in affinity chromatography for antibody purification. Protein A may be conjugated with various reporter molecules such as fluorescent dyes (FITC), enzyme markers (peroxidase, beta-galactosidase, alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates may be used to detect immunoglobulins using immunochemical assays such as western blotting, immunohistochemistry, and ELISA.
    
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- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    More than 90% of total protein
    
    Formulation
    
    The protein is solubilized in 20 mM Na-Phosphate pH 7.2, 150 mM NaCl.
    
    Storage
    
    Protein A is stable until the expiry date given on the label when stored at -70 °C. The protein can be kept at -20 °C for several weeks and on ice for several days. Repeated freezing and thawing should be avoided.
    
    Molecular Weight
    
    29.2 kDa
    
    * For Research Use Only
- Applications
  - Application Description
    
    Protein A was found to be a powerful reagent for the detection of IgG, and consequently the antigen against which these antibodies are directed. Protein A is widely used as a ligand coupled to resins in affinity chromatography for antibody purification. Protein A may be conjugated with various reporter molecules such as fluorescent dyes (FITC), enzyme markers (peroxidase, beta-galactosidase, alcaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates may be used to detect immunoglobulins using immunochemical assays such as western blotting, immunohistochemistry, and ELISA.

Datasheet

Recombinant Protein A, His-tagged

Recombinant Protein A, His-tagged

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