Name: Recombinant IL-6, Human, GMP
Price: 1445.00 USD

- Overview
  - Interleukin-6 (IL-6) is a pleiotropic, 22-28kDa cytokine which plays fundamental role in the acute phase response, inflammation, bone metabolism, lymphocyte differentiation and cancer progression. Deregulation of IL-6 production was also found in several diseases, including rheumatoid arthritis, Alzheimer’s disease, autoimmune deficiency disease and different types of cancer.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    interleukin 6, IFN-β2, B-Cell Differentiation Factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL6
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Reactivity
    
    Human
    
    Purification
    
    >98% as determined by SDS-PAGE analysis.
    
    Formulation
    
    Lyophilized from a 0.2µm filtered solution of PBS, pH 8.0.
    
    Storage
    
    Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
    
    Note
    
    Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
    
    Molecular Weight
    
    The protein has a calculated MW of 21.8kDa. The protein migrates as 22kDa under reducing condition (SDS-PAGE analysis).
    
    Class
    
    Cytokines
    
    Sequence
    
    MVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM with polyhistidine tag at the C-terminus.
    
    Affinity Tag
    
    His Tag (C-term)
    
    Endotoxin
    
    <0.05EU per 1μg of the protein by the LAL method.
    
    Activity
    
    Measure by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is <0.5ng/mL. The specific activity of recombinant human IL-6 is approximately >5x10^8 IU/mg. Measure by its ability to induce proliferation in MCF-7 cells. The ED50 for this effect is <4.4ng/mL.
    
    More Information
    
    UniProt
    
    P05231
    
    * For research use only.

Recombinant IL-6, Human, GMP

Recombinant IL-6, Human, GMP

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