Name: Recombinant SCF, Human, GMP
Price: 2013.00 USD

- Overview
  - Stem Cell Factor (SCF) is a 166-amino-acid protein with a monomeric molecular weight of approximately 18.5kDa. SCF is the ligand for the tyrosine kinase receptor c-kit, which regulates other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage-colony-stimulating factor (GM-CSF), and interleukin-3 to stimulate proliferation, and differentiation of hematopoietic stem cells. SCF can be produced by a variety of cells, including fibroblasts, smooth muscle, endothelial cells, mast cells and bone marrow macrophages endothelial cells.
    
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- Properties
  - Protein Name
    
    DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SF, SHEP7, SLF, KIT ligand
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Reactivity
    
    Human
    
    Purification
    
    >98% as determined by SDS-PAGE analysis.
    
    Formulation
    
    Lyophilized from a 0.2µm filtered solution of PBS, pH 7.4.
    
    Storage
    
    Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
    
    Note
    
    Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
    
    Molecular Weight
    
    The protein has a calculated MW of 19.4kDa. The protein migrates as 21kDa under reducing condition (SDS-PAGE analysis).
    
    Class
    
    Cytokines
    
    Sequence
    
    MEGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA with polyhistidine tag at the C-terminus
    
    Affinity Tag
    
    His Tag (C-term)
    
    Endotoxin
    
    <0.05EU per 1μg of the protein by the LAL method.
    
    Activity
    
    Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <5ng/mL. The specific activity of recombinant human SCF is >5x10^5 IU/mg.
    
    More Information
    
    UniProt
    
    P21583
    
    * For research use only.

Recombinant SCF, Human, GMP

Recombinant SCF, Human, GMP

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