Name: Recombinant Human tRNA-yW Synthesizing Protein 5
Price: Inquiry

- Overview
  - TYW5 Human Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 338 amino acids (1-315 a.a.) and having a molecular mass of 38.9kDa. TYW5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. tRNA wybutosine-synthesizing protein 5 (TYW5) functions as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position closest to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW). OHyW is a derivative of wybutosine observed in higher eukaryotes.
    
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- Properties
  - Protein Name
    
    tRNA wybutosine-synthesizing protein 5, hTYW5, TYW5, C2orf60.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 95.0% as determined by SDS-PAGE.
    
    Formulation
    
    TYW5 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
    
    Storage
    
    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Recombinant Human tRNA-yW Synthesizing Protein 5

Recombinant Human tRNA-yW Synthesizing Protein 5

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