Name: Recombinant EGF, Human, GMP
Price: 421.00 USD

- Overview
  - Human epidermal growth factor (EGF) is a 6kDa cytokine with 53 amino acid residues. EGF is mainly secreted from ectodermal cells, monocytes, kidney and duodenal glands. Upon binding to its receptor, EGFR, EGF acts to stimulate cell growth and proliferation of epithelial cells, play important roles in many developmental processes including accelerate tooth eruption, inhibits gastric acid secretion, and involve in wound healing.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    epidermal growth factor, Urogastrone, URG
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Reactivity
    
    Human
    
    Purification
    
    >97% as determined by SDS-PAGE analysis.
    
    Formulation
    
    Lyophilized from a 0.2µm filtered solution of PBS, pH 8.0.
    
    Storage
    
    Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
    
    Note
    
    Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
    
    Molecular Weight
    
    The protein has a calculated MW of 7.16kDa. The protein migrates as 9-11kDa under reducing condition (SDS-PAGE analysis).
    
    Class
    
    Cytokines
    
    Sequence
    
    MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus
    
    Affinity Tag
    
    His Tag (C-term)
    
    Endotoxin
    
    <0.05EU per 1μg of the protein by the LAL method.
    
    Activity
    
    Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <1.0ng/mL. The specific activity of recombinant human EGF is approximately >1.0x10^6 IU/mg.
    
    More Information
    
    UniProt
    
    P01133
    
    * For research use only.

Recombinant EGF, Human, GMP

Recombinant EGF, Human, GMP

More Information

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