Name: Recombinant GM-CSF, Human, GMP
Price: 1628.00 USD

- Overview
  - Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a growth factor due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    granulocyte-macrophage colony-stimulating factor, colony stimulating factor 2, CSF2, CSF-2
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Reactivity
    
    Human
    
    Purification
    
    >98% as determined by SDS-PAGE analysis.
    
    Formulation
    
    Lyophilized from a 0.2µm filtered solution of PBS, pH 8.0.
    
    Storage
    
    Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
    
    Note
    
    Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
    
    Molecular Weight
    
    The protein has a calculated MW of 15.4kDa. The protein migrates as 15kDa under reducing condition (SDS-PAGE analysis).
    
    Class
    
    Cytokines
    
    Sequence
    
    APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE with polyhistidine tag at the N-terminus.
    
    Affinity Tag
    
    His Tag (N-term)
    
    Endotoxin
    
    <0.05EU per 1μg of the protein by the LAL method.
    
    Activity
    
    Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <80pg/mL. The specific activity of recombinant human GM-CSF is approximately >1x10^7 IU/mg.
    
    More Information
    
    UniProt
    
    P04141
    
    * For research use only.

Recombinant GM-CSF, Human, GMP

Recombinant GM-CSF, Human, GMP

More Information

We Also Recommend

Recombinant IFN gamma, Human, GMP

Recombinant IL-1 beta, Human, GMP

Recombinant IL-10, Human, GMP