Name: Recombinant 4-1BBL, Human, GMP
Price: 2100.00 USD

- Overview
  - 4-1BB ligand (4-1BBL) is a type II transmembrane protein that is part of the tumor necrosis factor (TNF) ligand family. As an inducible co-stimulatory molecule, it presents on several antigen presenting cell (APC) types, including B cells, macrophages and DCs. The interactions between 4-1BB and 4-1BBL trigger pleiotropic effects on the immune response including antigen presenting process, proliferation of CD4 and CD8 positive T-cells, as well as cytokine secretion from T-cells through NFκB, c-Jun, and p38 downstream signal pathways activation. Therefore, 4-1BB and 4-1BBL are recently used for the immunotherapy of cancer.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    4-1BB ligand, CD137L, TNLG5A, TNFSF9, Tumor necrosis factor ligand superfamily member 9
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Reactivity
    
    Human
    
    Purification
    
    >95% as determined by SDS-PAGE analysis.
    
    Formulation
    
    Lyophilized from a 0.2µm filtered solution of PBS, pH 7.4.
    
    Storage
    
    Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
    
    Note
    
    Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
    
    Molecular Weight
    
    The protein has a calculated MW of 20.4kDa. The protein migrates as 20kDa under reducing condition (SDS-PAGE analysis).
    
    Class
    
    Cytokines
    
    Sequence
    
    MREGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPRSE with polyhistidine tag at the C-terminus.
    
    Affinity Tag
    
    His Tag (C-term)
    
    Endotoxin
    
    <0.05 EU per 1μg of the protein by the LAL method.
    
    Activity
    
    Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is 1-5ng/mL.
    
    More Information
    
    UniProt
    
    P41273
    
    * For research use only.

Recombinant 4-1BBL, Human, GMP

Recombinant 4-1BBL, Human, GMP

More Information

We Also Recommend

Recombinant Activin A, Human, GMP

Recombinant EGF, Human, GMP

Recombinant FGF-2 (aa 135-288.), Human, GMP