Rapid Detection Kit for His-tagged Proteins (Highly Sensitive Competitive Assay)

The Rapid Detection Kit is used for quick, simple detection of His-tagged proteins (1 ug/ml - 10 ug/ml) through a highly sensitive colloidal gold-based lateral flow immunoassay (LFIA). The LFIA strip is composed of membrane immobilized antibodies, sample pad, conjugate pad, and absorbent pad.
The gold conjugated capture antibodies are immobilized at conjugate pad. The His-tagged proteins are immobilized over test line. The secondary antibodies are immobilized at control zone. Upon application of a sample to the strip, the gold conjugated capture antibody binds to the His-tag present in the sample while flowing across the membrane. If no His-tagged protein is present in the sample or the concentration of His-tagged proteins is below detectable levels, the free gold conjugated capture antibodies will bind to the His-tagged proteins embedded at the detection zone and form a red line, representing a negative result. Intensity of color at test line is inversely related to the concentration of His-tagged proteins in the sample. In both positive and negative tests, the gold-conjugated anti-His capture antibodies will bind to the secondary antibodies immobilized at the control line. A red control line will appear in both positive and negative results.
The color of the test line is negatively correlated with the concentration of His-tagged proteins in the sample. When the concentration of His-tagged proteins in the sample reaches 1 ug/ml and above, the intensity of the test line will be effectively weakened, showing a light red band or no color. The lateral flow strip will continue to show positive results at concentrations greater than 10 ug/ml, visualized by complete loss of the test line.

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