Rapid Detection Kit for His-tagged Proteins (Competitive Assay)

The Rapid Detection Kit is used for quick, simple detection of His-tagged proteins through a sensitive colloidal gold-based lateral flow immunoassay (LFIA). The LFIA strip is composed of membrane immobilized antibodies, sample pad, conjugate pad, and absorbent pad.
The gold conjugated capture antibodies are immobilized at conjugate pad. The His-tagged proteins are immobilized over test line. The secondary antibodies are immobilized at control line section. The sample is applied to the sample pad at one end of the strip and it subsequently migrates to the other parts of strip by capillary action. If the sample contains His-tagged proteins, the gold conjugated capture antibodies will bind to these His-tagged proteins in the sample and not to the immobilized His-tagged proteins in the test zone. Intensity of color at test line inversely corresponds to the number of His-tagged proteins in the sample. In a sample where no His-tagged proteins are present or the concentration of His-tagged proteins is below detectable levels, the free gold-conjugated capture antibodies will bind to the His-tagged proteins embedded on the test line and form a red line, representing a negative result. In both positive and negative tests, the gold-conjugated anti-His-tag antibodies will bind to the secondary antibodies immobilized at the control line. A red control line will appear in both positive and negative results.
The color of the test line is negatively correlated with the concentration of His-tagged proteins in the sample. The recommended detection range for this kit is 10 ug/ml to 100 ug/ml of His-tagged proteins. When the concentration of His-tagged proteins in the sample reaches 10 ug/ml and above, the intensity of the test line will be effectively weakened, showing a light red band or no color. The lateral flow strip will continue to show positive result at concentrations greater than 100 ug/ml, visualized by complete loss of the test line.

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