Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains)

Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains)

Catalog Number:
A001007005NOR
Mfr. No.:
RARa/Ig/7S
Price:
$498
  • Size:
    10 mg
    Quantity:
    Add to Cart:
      • Overview
        • Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

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          Background

          The defined antibody specificity is directed to the major isotypes of the rat immunoglobulin system (classes, subclasses and L chain types) including antibody to common determinants, to class and subclass-specific determinants, and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

      • Properties
        • Host
          rabbit
          Antibody Type
          polyclonal
          Formulation
          Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity
          Storage
          The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
          Note
          This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

          * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

      • Applications
        • Application Description
          Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting.

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