Rabbit Anti-Plum Pox Virus (PPV) IgG fraction POLYCLONAL

Rabbit Anti-Plum Pox Virus (PPV) IgG fraction POLYCLONAL

Catalog Number:
A001277681AUS
Mfr. No.:
AUST_AP-10103-9
Price:
$760
  • Size:
    1mg
    Quantity:
    Add to Cart:
      • Overview
        • IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mixture of three synthetic peptides corresponding to aa86-96 (STGTFTVPRLK), aa146-158 (KRDYDVTDDEMSI), and aa242-255 (SLARYAFDFYEMTS) of the PPV coat protein. The IgG fraction was purified using Protein G-Sepharose.

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      • Properties
        • Antibody Type
          polyclonal
          Formulation
          Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
          Storage
          Store at -20°C.

          * For research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

      • Applications
        • Application Description
          This polyclonal antibody reacts with extracts of PPV-infected plants in DAS-ELISA as a capture antibody (1: 2,000 dilution) and Western blot assay (1: 500 dilution). PPV belongs to the genus Potyvirus of the family Potyviridae and is the causal agent of sharka disease of stone fruits crops. PPV genome consists of a single-stranded, positive-sense RNA encapsidated by a single glycosylated coat protein (34 kDa). This protein is also involved in systemic movement of the virus. PPV has flexuous filamentous morphology and is transmitted by an insect and grafting.
      • Reference
        • Fernández-Fernández, M.R. et. al. (2002) J. Biol. Chem. 277, 135-140.
          Riechmann, J.L. et. al. (1992) J. Gen. Virol. 73, 1-6.
          Laín, S. et. al. (1989) Virus Res. 13, 157-172.

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