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Overview
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Delipidated, heat inactivated, lyophilized, stable whole serum. Sodium azide 1 mg/ml. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
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Background
The defined antibody activity is directed to the various forms of thrombospondin. In bidimensional electrophoresis a single precipitin line is obtained with purified thrombospondin; with platelet lysate concentrate a slight precipitin line becomes visible. No precipitation is obtained with normal human serum. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion Mancini, Ouchterlony), electroimmunodiffusion (Laurell) and neutralization assay. The presence of non-precipitating antibodies has not been assayed. This does not exclude use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
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Overview