Rabbit anti Human alpha-1 foetoprotein, conjugated with FITC

Rabbit anti Human alpha-1 foetoprotein, conjugated with FITC

Catalog Number:
A001006115NOR
Mfr. No.:
RAHu/AFP/FITC
Price:
$440
  • Size:
    1 ml
    Quantity:
    Add to Cart:
      • Overview
        • FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

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          Background

          The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against amniotic fluid, hepatoma sera and purified alpha-foetoprotein a single precipitin line is obtained. No precipitation reaction is obtained with normal human serum. To identify and measure alpha foetoprotein at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

      • Properties
        • Host
          rabbit
          Antibody Type
          polyclonal
          Formulation
          FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
          Storage
          The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
          Note
          This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

          * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

      • Applications
        • Application Description
          ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence.

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