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Overview
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Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
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Background
The reactivity of the antiserum is restricted to lactoperoxidase as tested in immunoelectrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma. In immunocytochemical and immunohistochemical staining to identify and measure lactoperoxidase at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
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- Properties
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Overview