QUANTIFICATION Circulating DNA

QUANTIFICQuantification of cfDNA is ideally carried out by qPCR or capillary electrophoresis since common methods such as absorption measurement or fluorescent dye based quantification might lead to false results due to low DNA concentration.

The total cf DNA isolated can be quantified using the Cell-free human DNA detc-qPCR Test designed to target a conserve sequence region of a gene repeated more than a hundred times in the human genome.

Are individuals ready-to-use tubes containing all the components needed to perform the quantitative PCR assay.ATION Circulating DNA

Specifications:Real-time PCR amplification plot for cfhDNA dtec-qPCR Test (red) targeting a “non-truncated” multi-copy gene and compared to a monocopy target (blue), using a human genomic DNA as a standard. Due to the presence of multiple copies of the selected target, sensibility is increased 2 logs (100 times) for the cfhDNA dtec-qPCR Test. Same increased signal is observed for the purified cell-free DNA samples employed for cell-free DNA quantification.

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