QuantiChrom™ Hyaluronidase Inhibitor Screening Assay Kit

HYALURONIDASES are a family of enzymes that catalyze the degradation of the glycosaminoglycan, hyaluronic acid. Hyaluronic acid is one of the major constituents of the extracellular matrix in organisms where it contributes to both cell proliferation and migration. The role of hyaluronidases in breaking down this key factor in cell proliferation makes them a possible target for cancer treatment. One hypothesis is that increased hyaluronidase may help prevent tumor invasion by breaking down the extracellular matrix needed for tumor expansion. Conversely, decreasing hyaluronidase activity might prevent metastasis by stopping cancer cells from escaping primary tumor masses. The study to determine hyaluronidases’ exact role in cancer pathology is still ongoing. Our Hyaluronidase Inhibitor Screening Assay Kit uses a two step turbidimetric reaction to measure hyaluronidase activity by the amount of hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction and forms turbidity with any residual hyaluronic acid in the well. The decrease in turbidity at 600 nm is directly proportional to hyaluronidase activity in the sample.

Key Features
▪ Rapid and reliable. Entire assay can be completed in 30 min.
▪ Simple and Convenient. Simple procedure with an enzymatic reaction and addition of stop reagent. No wash or reagent transfer steps involved.
▪ Robust and amenable to HTS. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

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