QuantiChrom™ BCG Albumin Assay Kit

- Overview
  - Albumin is the most abundant plasma protein in human. It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure, binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium and magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition and protein-losing enteropathies. High levels are associated with dehydration. Simple, direct and automation-ready procedures for measuring albumin concentration in biological samples are becoming popular in Research and Drug Discovery. Our BCG albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol green that forms a colored complex specifically with albumin. The intensity of the color, measured at 620nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
    
    Key Features
    ▪ Sensitive and accurate. Use as little as 5 µL samples. Detection range 0.01 – 5 g/dL (1.5 – 750 µM) albumin in 96-well plate assay.
    ▪ Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
    ▪ Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
    ▪ No interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Method: OD620nm (BCG)
    Samples: Serum, plasma, urine, biological preparations
    Species: All
    Procedure: 5 min
    Detection Limit: 0.01 g/dL
    Shelf Life: 12 months
    
    Storage
    
    4, -20°C
    
    * For Research Use Only
- Applications
  - Application Description
    
    For quantitative determination of albumin and evaluation of drug effects on albumin metabolism.
- Reference
  - Zhao, C., et al(2020). Cyclin G2 regulates canonical Wnt signalling via interaction with Dapper1 to attenuate tubulointerstitial fibrosis in diabetic nephropathy. Journal of Cellular and Molecular Medicine, 24(5), 2749-2760. Assay: Albumin in Mice Urinary Albumin.
    Xu, D., et al (2018). NIX-mediated mitophagy protects against proteinuria-induced tubular cell apoptosis and renal injury. American Journal of Physiology-Renal Physiology, 316(2) Assay: Albumin in Mice Urinary Albumin.
    Manna, L., et al (2020). Impact of Phyllantus niruri and Lactobacillus amylovorus SGL 14 in a mouse model of dietary hyperoxaluria. Beneficial Microbes, 11(6), 547-559. Assay: Albumin in Mice Kidney Tissue.
    Sethi, P., et al (2019). Plasma protein and lipoprotein binding of cis- and trans-permethrin and deltamethrin in adult humans and rats. Drug Metabolism and Disposition: The Biological Fate of Chemicals, 47(9), 941-948. Assay: Albumin in Rat and Human Plasma.
    Park, S.-J., et al (2019). Discovery of endoplasmic reticulum calcium stabilizers to rescue ER-stressed podocytes in nephrotic syndrome. Proceedings of the National Academy of Sciences, 116(28), 14154-14163. Assay: Albumin in Mice Urine.
    Amor, C., et al (2020). Senolytic CAR T cells reverse senescence-associated pathologies. Nature, 583(7814), 127-132. Assay: Albumin in Murine Serum.
    Parchem, J. G., et al (2021). STOX1 deficiency is associated with renin-mediated gestational hypertension and placental defects. JCI Insight, 6(2). Assay: Albumin in Mice Urine Albumin.
    Liu, X., et al (2019). Metabolomics analysis reveals the protection mechanism of huangqi-danshen decoction on adenine-induced chronic kidney disease in rats. Frontiers in Pharmacology, 10. Assay: Albumin in Male Sprague-Dawley Rats Urine.
    Blom, E. (2019). Receiving and backgrounding phase management: The effects of receiving diet roughage source and backgrounding phase rate of gain on performance of feedlot cattle. Electronic Theses and Dissertations. Assay: Albumin in Angus-based crossbred steer calves Blood.
    Proffitt, S., Curnow, E., Brown, C., Bashir, S., & Cardigan, R. (2018). Comparison of automated and manual methods for washing red blood cells. Transfusion, 58(9), 2208-2216. Assay: Albumin in red blood cells.
    Rangel, E. B., Gomes, S. A., Kanashiro-Takeuchi, R., Saltzman, R. G., Wei, C., Ruiz, P. & Hare, J. M. (2018). Kidney-derived c-kit+ progenitor/stem cells contribute to podocyte recovery in a model of acute proteinuria. Scientific reports, 8(1), 14723. Assay: Albumin in rats blood.
    Shepardson, K. M., Larson, K., Johns, L. L., Stanek, K., Cho, H., Wellham, J. & Rynda-Apple, A. (2018). IFNAR2 is required for anti-influenza immunity and alters susceptibility to post-influenza bacterial superinfections. Frontiers in Immunology 9:2589. Assay: Albumin in mice cell free BAL fluid.
    Xu, D. et al(2018). NIX-mediated mitophagy protects against proteinuria-induced tubular cell apoptosis and renal injury. American Journal of Physiology-Renal Physiology, 316(2), F382-F395. Assay: Albumin in mice epithelial cells.
    Chuang, M. H., Jan, M. S., Chang, J. T., & Lu, F. J. (2017). The Chinese medicine JC-001 enhances the chemosensitivity of Lewis lung tumors to cisplatin by modulating the immune response. BMC complementary and alternative medicine, 17(1), 210. Assay: Albumin in human venous blood.
    Garnier, R., Bento, A. I., Hansen, C., Pilkington, J. G., Pemberton, J. M., & Graham, A. L. (2017). Physiological proteins in resource-limited herbivores experiencing a population die-off. The Science of Nature, 104(7-8), 68. Assay: Albumin in sheep plasmatic albumin.
    Garnier, R., Cheung, C. K., Watt, K. A., Pilkington, J. G., Pemberton, J. M., & Graham, A. L. (2017). Joint associations of blood plasma proteins with overwinter survival of a large mammal. Ecology letters, 20(2), 175-183. Assay: Albumin in sheep plasmatic albumin.
    Hassanein, W. et al (2017). Liver Scaffolds Support Survival and Metabolic Function of Multilineage Neonatal Allogenic Cells. Tissue Engineering Part A, 24(9-10), 786-793. Assay: Albumin in rats liver cells.
    Hassanein, W., Uluer, M. C., Langford, J., Woodall, J. D., Cimeno, A., Dhru, U. & Khalifeh, A. (2017). Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold. Organogenesis, 13(1), 16-27. Assay: Albumin in human liver cells.
    Kingman, J., Uitto, J., & Li, Q. (2017). Elevated dietary magnesium during pregnancy and postnatal life prevents ectopic mineralization in Enpp1asj mice, a model for generalized arterial calcification of infancy. Oncotarget, 8(24), 38152. Assay: Albumin in mice urinary albumin.
    Van Le, T., Nguyen, N. H., Do, H. Q., Le, H. M., & Truong, N. H. (2017). Transplantation of umbilical cord blood-derived mesenchymal stem cells to treat liver cirrhosis in mice: a comparison of tail and portal vein injection. Progress in Stem Cell, 4(2), 201-216. Assay: Albumin in human liver cells.
    Blair, T. C., Manoharan, M., Rawlings-Rhea, S. D., Tagge, I., Kohama, S. G., Hollister-Smith, J. & Rooney, W. D. (2016). Immunopathology of Japanese macaque encephalomyelitis is similar to multiple sclerosis. Journal of neuroimmunology, 291, 1-10. Assay: Albumin in macaque .
    Frej, C., Linder, A., Happonen, K. E., Taylor, F. B., Lupu, F., & Dahlback, B. (2016). Sphingosine 1-phosphate and its carrier apolipoprotein M in human sepsis and in Escherichia coli sepsis in baboons. Journal of cellular and molecular medicine, 20(6), 1170-1181. Assay: Albumin in human, baboon albumin.
    Fujino, T., & Hasebe, N. (2016). Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy. BMC nephrology, 17(1), 179. Assay: Albumin in mouse cells.
    Lei, G. S., Zhang, C., Zimmerman, M. K., & Lee, C. H. (2016). Vitamin D as supplemental therapy for Pneumocystis pneumonia. Antimicrobial agents and chemotherapy, 60(3), 1289-1297. Assay: Albumin in mice cell free BAL fluid.
    Sethi, P. K., White, C. A., Cummings, B. S., Hines, R. N., Muralidhara, S., & Bruckner, J. V. (2016). Ontogeny of plasma proteins, albumin and binding of diazepam, cyclosporine, and deltamethrin. Pediatric research, 79(3), 409. Assay: Albumin in human plasmatic albumin.
    Truong, N. H., Nguyen, N. H., Le, T. V., Vu, N. B., Huynh, N., Nguyen, T. V. & Pham, P. V. (2016). Comparison of the treatment efficiency of bone marrow-derived mesenchymal stem cell transplantation via tail and portal veins in CCl4-induced mouse liver fibrosis. Stem Cells International, 2016. Assay: Albumin in mice venous blood.
    Tsang, G., Insel, M. B., Weis, J. M., Morgan, M. A. M., Gough, M. S., Frasier, L. M. & Pietropaoli, A. P. (2016). Bioavailable estradiol concentrations are elevated and predict mortality in septic patients: a prospective cohort study. Critical Care, 20(1), 335. Assay: Albumin in human venous blood.
    Yokoyama, S., Hiramoto, K., Koyama, M., & Ooi, K. (2016). Chronic liver injury in mice promotes impairment of skin barrier function via tumor necrosis factor-alpha. Cutaneous and ocular toxicology, 35(3), 194-203. Assay: Albumin in mice plasmatic albumin.
    Doi M, Sultana Rekha R, Ahmed S, Okada M, Kumar Roy A, El Arifeen S, Ekstrom EC, Raqib R, Wagatsuma Y. (2011). Association between calcium in cord blood and newbornsize in Bangladesh. Br J Nutr. 106(9):1398-407. Assay: Albumin in human blood.
    Euhus DM, et al (2011). Tamoxifen Downregulates Ets-oncogene Family Members ETV4 and ETV5 in Benign Breast Tissue: Implications for Durable Risk Reduction. Cancer Prev Res (Phila). 4(11):1852-62. Assay: Albumin in human blood.
    Zhang G, et al (2011). Nicotinic acetylcholine receptor alpha1 promotes calpain-1 activation and macrophage inflammation in hypercholesterolemic nephropathy. Lab Invest. 91(1):106-23. Assay: Albumin in mouse urine.
    Irani K, et al (2010). Mechanical dissociation of swine liver to produce organoid units for tissue engineering and in vitro disease modeling. Artif Organs. 34(1):75-8. Assay: Albumin in swine hepatocyte.
    Roberts DJ, et al (2009). Effect of acute inflammatory brain injury on accumulation of morphine and morphine 3- and 6-glucuronide in the human brain. Crit Care Med. 37(10):2767-74. Assay: Albumin in human plasma and cerebrospinal fluid.
    Cosgrove, D. et al (2008). Integrin alpha1beta1 Regulates Matrix Metalloproteinases via P38 Mitogen-Activated Protein Kinase in Mesangial Cells. Implications for Alport Syndrome. Am. J. Pathology 172(3):761-73. Assay: Albumin in mouse urine.
    Helsby, NA et al (2008). CYP2C19 pharmacogenetics in advanced cancer: compromised function independent of genotype. Br J Cancer. 99(8):1251-5. Assay: Albumin in human serum.
    Kain R, et al (2008). Molecular mimicry in pauci-immune focal necrotizing glomerulonephritis. Nat Med. 14(10):1088-96. Assay: Albumin in human albuminuria.
    Osborn TM, et al (2008). Decreased levels of the gelsolin plasma isoform in patients with rheumatoid arthritis. Arthritis Res Ther. 10(5):R117. Assay: Albumin in human plasma and synovial fluid.
    Sullivan JP, et al (2008). Convection and hemoglobin-based oxygen carrier enhanced oxygen transport in a hepatic hollow fiber bioreactor. Artif Cells Blood Substit Immobil Biotechnol.36(4):386-402. Assay: Albumin in human hepatocyte.
    Lee, R.H. et al (2006) Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD_scid mice. PNAS 103 (46): 17438-17443. Assay: Albumin in mouse urine.
    Rebecca R. (2006). Associations of histories of depression and PMDD diagnosis with allopregnanolone concentrations following the oral administration of micronized progesterone sychoneuroendocrinology 31(10):1208-1219. Assay: Albumin in human serum.
    Wang S, et al (2006). Renal bone morphogenetic protein-7 protects against diabetic nephropathy. J Am Soc Nephrol. 17(9):2504-12. Assay: Albumin in mouse serum.
    Zhang Q, et al (2006). Serum and mucosal antibody responses to pneumococcal protein antigens in children: relationships with carriage status. Eur J Immunol.36(1):46-57. Assay: Albumin in human saliva and serum.

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QuantiChrom™ BCG Albumin Assay Kit

QuantiChrom™ BCG Albumin Assay Kit

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