PythoN® Tissue Dissociation System

The Singleron PythoN® Automated Tissue Dissociation System combines mechanical and enzymatic dissociation. The streamlined workflow quickly dissociates the tissue into high-quality single-cell suspension. Equipped with broad-spectrum sCelLive® Tissue Dissociation Mix and disposable Singleron PythoN® Dissociation Units, the Singleron PythoN® Tissue Dissociator can automatically process tissues to obtain highly viable and high-quality single-cell suspensions. The precise digital controling module enables a quick exploration of the best dissociation conditions for a specific tissue type.
Singleron PythoN® was used to process murine liver tissues in triplicates obtaining single cell suspensions with >85% cell viability. Cell clustering and cell type ratios were consistent across the three replicates, showing high stability and reproducibility. PythoN® processes 14 mg and 33 mg mouse liver tissues, achieving high cell viability and yield. This breakthrough allows integration of clinical needle biopsies into single-cell sequencing workflows.
Singleron PythoN® is suitable for low-input samples, even needle biopsies.

System Highlights:
• Gentle by design, specifically for single cell
• Efficiently processes up to 8 samples in 15 min at high viability
• Flexible sample sizes from needle biopsies to 2 g
• Fully customizable plug-and-play suite of tested protocols

Technical Specifications:
• Dimensions: 365mm x 325mm x 360mm
• Weight: 10 kg
• Capacity: Up to 8 samples / run
• Time: 15 min / standard
• Flexible: 50 programs
• Temperature: At RT or controlled at 37°C

Please contact us at for specific academic pricing.