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Overview
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pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency. The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.
Includes for 20 rxn:
– 20 µL pSpark® I (20 ng/µL)
– 20 µL T4 DNA Ligase (5U/Weiss)
– 200 µL T4 DNA Ligase Buffer (5x)
– 150 µL PEG 6000 (10x)
– 5 µL Insert Control 1 kb (20 ng/µL)Please contact us at for specific academic pricing.
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Properties
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Storage-20 °C (NON Frost-Free Freezer).
* This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
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Applications
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Application DescriptionGeneral cloning.
Cloning of High Fidelity PCR amplified products.
Production of ssDNA.
Blue/white screening for recombinants.
In vitro transcription from T7/SP6 dual-opposed promoters.
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Reference
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Overview