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Overview
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pSpark® Done is a highly efficient, accurate and easy-to-use DNA Cloning system designed for cloning of blunt ended DNA with very high efficiency. The MCS of the pSpark® Done vector incorporates sequences on either side of the insert that are recognized by the restriction enzymes NotI and EcoRI. This allows the insert DNA to be removed with a single restriction digest using either of these enzymes
Includes for 20 rxn:
– 20 µL pSpark® Done (20 ng/µL)
– 20 µL T4 DNA Ligase (5U/Weiss)
– 200 µL T4 DNA Ligase Buffer (5x)
– 150 µL PEG 6000 10x
– 5 µL Insert Control 1 kb (20 ng/µL)Please contact us at for specific academic pricing.
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- Properties
- Applications
- Reference
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Overview