Pseudorabies Virus (PRV) for Neuronal Tracing

Retrograde, multisynaptic
Pseudorabies virus (PRV) have a double-stranded DNA genome, and the genome is approximately 150 kb that encode capsid, tegument, and envelope protein. The retrograde trans-synaptic PRV is modified from vaccine strain Bartha, which can be used to analyze the structure of neuronal connectivity in brain center and periphery. After the virus infects nerve cells, it can replicates and expresses the target gene in the cell. The progeny viruses are transported to the synapse and then retrograde trans-synaptic labeling of the upstream neuron for further replication and transmission across the synapse.

Advantages
· Fast and high efficiency
· Strong background in neuronal tracing tool
· Hypotoxicity and strong signal
· The most competitive price

Please contact us at for specific academic pricing.

Background

The neuronal connectivity by different synapses forms the brain's complex activities. So developing the technology to dissect the brain circuits became urgent in neuroscience to understand the brain functions. Currently, techniques for studying brain circuits include conventional tracers and recombinant viral vectors. The conventional tracers like CTB, HRP, PHA-L, BDAs and so on, but they have some disadvantages, such as:
1) Cell specificity cannot be achieved.
2) Efficiency is not stable.
3) The circuits of the neurons at multiple step of synaptic connections cannot be realized.

However, due to the ability to efficiently enter cells and deliver a variety of gene, genetically engineered recombinant viral vectors emerged as powerful tools for visualizing neural connectivity. The commonly used neurotropic virus, such as HSV (Herpes simplex virus type 1), VSV (Vesicular stomatitis virus), PRV (pseudorabies virus) and RABV (rabies virus) can cross synapse from one cell to another, if we provide the protein that used for replicate. In addition, there are also SFV (mark the fine morphology of in-situ neurons) and AAV viruses(as a helper virus to express exogenous genes or as a monosynaptic tracer with serotypes 1) that are used for neuronal tracing.