Protein MMAE Conjugation Kit

This personalized conjugation kit works with any protein with a MW over 20 KDa. The customer supplies their own unmodified protein. Using the kit components, the customer converts their protein to a thiol-protein, followed by reaction of the maleimide-activated VC-PAB-MMAE to generate the protein-MMAE conjugates (a similar method developed by Seattle Genetics: Sun et al.2005,Bioconjugate Chem. 16, 1282-1290). A One-step purification typically provides the resulting protein-MMAE conjugate at greater than 95% purity.

This kit provides materials to conjugate 6.67-20 nmol of one (CM11413x1) or three protein samples (CM11413x3).

If you have smaller amount of protein, a protein with a MW below 20KDa, or other customization needs, please select the customization radio button and/or fill out the information above.

• Requirement for protein:
Amount: 6.67-20 nmol
MW: >20 KDa
Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
Protein has some lysine residues

• Drug Information
Name: Monomethyl auristatin E (MMAE) with Mal-Val-Cit-PAB linkage
CAS number: 646502-53-6
Mechanism of action: Inhibits cell division by blocking the polymerization of tubulin. VC-PAB linker is stable in extracellular fluid, but cleaved by cathepsin B once inside the tumor cell, thus activating the antimitotic mechanism
Activities: Antioxidant, anti-inflammatory, anticancer, and insecticidal activities

• Key Features of this PerKit™
Offers a simple and easy way to label protein with MMAE with minimum exposure to the toxin
Cathepsin B cleavable VC-PAB (Ref. Doronina et. al. 2008, Bioconjugate Chem. 19, 1960-1963)
Fast and easy preparation: 6 h preparation and <2 h hands-on time
All reagents and supplies included for preparation and purification
Options to choose tailored services after conjugation

Please contact us at for specific academic pricing.

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