Protein G Bestarose 4FF - Antibody Affinity Resins

Antibody Affinity Resins

Appearance: White slurry
Matrix: Highly cross-linked agarose, 4%
Particle size +: 45-165μm
Functional group: Protein G (E.coli)
Ligand concentration: ~2mg Protein G /mL resin
Cross-linking method: Cyanogen bromide activation
Dynamic binding capacity++: ~20mg human IgG /mL packed resin
Chemical stability: 40°C,1 week: 1M HAc (pH2.0),20mM sodium phosphate, 1%SDS, 6M GuHCl, 70% ethanol
At room temperature 2h: 0.1 M HCl (pH1.0), 8M Urea (pH10.5)
Physical stability: Volume changes due to changes in pH or ionic strength are negligible
Max. pressure: 0.3MPa
Pressure flow velocity: 150~250cm/h (0.1MPa BXK50, H=25cm)
pH stability: 3~9 (working), 2~10 (CIP)
Storage+++: 2~8°C,20% ethanol or 2% benzyl alcohol

+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total
++ Linear flow velocity 200cm / h, bed height 10cm, buffer conditions: 20mM PB, 0.15M NaCl, pH 7.4
+++2% benzyl alcohol is only used for international transport or special requirements from customer.