Protein A/G Coated - 96 well Solid plates

Protein A/G Plates 96-Well Solid Plates: Solid format designed for high-performance assays

Protein A/G Coated Plates provide an efficient alternative to traditional passive adsorption methods for immobilizing antibodies in ELISA assays. The surface of these plates is designed to bind a wide range of IgG species, including all subclasses of mouse IgG (except IgA, IgM, or serum albumin) and all subclasses of human IgG, as well as IgA, IgE, IgM, and IgD. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, offering versatility in binding different immunoglobulins. Unlike Protein A alone, Protein A/G has reduced pH dependency, while combining the beneficial properties of both Protein A and Protein G, making it a powerful tool for various immunoassays.

Protein A/G Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Protein A/G Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Protein A/G Coated Surface on Protein A/G Coated 96 Well Solid Plates is designed to bind a wide range of IgG species, including all subclasses of mouse IgG (except IgA, IgM, or serum albumin) and all subclasses of human IgG, as well as IgA, IgE, IgM, and IgD. This versatile product is ideal for the purification and detection of mouse monoclonal IgG antibodies, as well as the purification of macaque IgG, offering a powerful tool for immunoassays and antibody-based applications.

Key features of Protein A/G Plates
· Protein A/G surface: It binds a wide range of IgG species, including all subclasses of mouse IgG (except IgA, IgM, or serum albumin) and all subclasses of human IgG, as well as IgA, IgE, IgM, and IgD
· specific and sterically oriented bond of antibodies
· purification and detection of mouse monoclonal IgG antibodies and for purification of macaque IgG
· highest specificity and capacity
· retains antibody activity and orients antibody for maximum binding
· generally not suitable for sandwich ELISA assays
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Protein A/G Coated Surface provides an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.
Protein A/G Coated Surface is a surface that binds to most IgG species (in particular binds to all subclasses of mouse IgG excluding mouse IgA, IgM or serum albumin and to all subclasses human IgG), IgA, IgE, IgM and IgD.

Protein A/G includes four Fc binding domains from Protein A and two from Protein G making it a versatile tool. The binding dependency to pH of Protein A/G is lower than Protein A but has the additive properties of Protein A and G together.

Protein A/G binds to human IgG, IgA, IgM; it binds to all subclasses of mouse IgG excluding mouse IgA, IgM and serum albumin (see Table 1). When coated onto microplates, the Protein A/G can securely be used in purification and detection of mouse monoclonal IgG antibodies with no interference from IgA, IgM and serum albumin.

· Examples of applications for Protein A/G Coated surface
specific and sterically oriented bond of antibodies
highest specificity and capacity
retains antibody activity and orients antibody for maximum binding
generally not suitable for sandwich ELISA assays

· Protein A/G Coated Surfaces main features
Biomat Protein A/G Coated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

Also, Protein A/G Coated Products ensures:

Uniformity
The Protein A/G Coated Products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability
The Protein A/G Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Protein A/G Coated Microplates Specifications
Coating
Recombinant Protein A/G (M.W. 50.4 kDa) is a fusion protein between Protein A and Protein G. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C and the Protein G portion is from Streptococcus sp. segments C1 and C3, expressed in E. coli. Protein A/G is coated using 200 µl/well.

Binding capacity
Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.

Protein A/G Coated PCR 8 Strip Tubes and PCR Plates Specifications
Coating
Recombinant Protein A/G (M.W. 50.4 kDa) is a fusion protein between Protein A and Protein G. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C and the Protein G portion is from Streptococcus sp. segments C1 and C3, expressed in E. coli. Protein A/G is coated using 100 µl/tube.