-
-
Overview
-
Concanavalin A Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.
Concanavalin A Coated Plates are designed for the specific binding of carbohydrate fractions in glycoproteins, enzymes, and cell membranes. Derived from Canavalia ensiformis (jack bean), Concanavalin A is a lectin commonly used for isolating glyco-conjugates and glycoproteins with specific carbohydrate structures. These plates provide a highly sensitive platform for ELISA assays and are ideal for applications involving glycoproteins, glycopeptides, enzyme-antibody conjugates, as well as interactions with polysaccharides, glycolipids, cell membranes, hormones, and hormone receptors. Their versatility makes them a reliable tool for assays focused on carbohydrate-protein interactions.
Concanavalin A Coated 96 Well Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.
Concanavalin A Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.
Surface:
The Concanavalin A surface provide a highly sensitive and specific platform for binding the carbohydrate fractions of glycoproteins, enzymes, and cell membranes. The Concanavalin A plates are ideal for applications such as interactions with glycoproteins, glycopeptides, polysaccharides, and enzymatic-antibody conjugates. Additionally, they support binding with cell membranes, hormones, and hormone receptors, making them a versatile tool for a variety of biochemical and cellular assays.
Key features of Concanavalin A Plates
· Concanavalin A Surface:
· Specifically binds glycoproteins, glycopeptides, and enzyme-antibody conjugates
· Supports interactions with polysaccharides and glycolipids for versatile applications
· Facilitates binding to cell membranes, hormones, and hormone receptors
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 75 to 200 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use
Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.Please contact us at for specific academic pricing.
Background
Concanavalin A Coated Surface offers a powerful and sensitive instrument for binding in specific way the carbohydrate fraction of glycoproteins, enzymes and cell membranes.
Concanavalin A Coated Surface shows specific affinity for molecules containing α-D-mannopyranosyl, α-D-glucopyrannosyl and sterically related and binds in specific way the carbohydrate fraction of glycoproteins, enzymes and cell membranes.
Concanavalin A, belonging to the lectins family, is a Hemagglutinin from the common jack bean Canavalia ensiformis. It is well known that lectins have been used extensively for the isolation of glyco-conjugates and glycoproteins with specific carbohydrate structures.
The optical properties of polystyrene remain unchanged, allowing the use of the modified surface as a powerful tool for diagnostic assays.
· Examples of applications for Concanavalin A Coated surface
interaction with glycoproteins, glycopeptides and enzyme-antibody conjugates
polysaccharides and glycolipids
interaction with cellular membranes, hormones and hormone receptors
· Concanavalin A Coated Surfaces main features
Biomat Concanavalin A Coated products have the following characteristics:
Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
· Also, Concanavalin A Coated Products ensures:
Uniformity
The Concanavalin A Coated Products show a CV% less than 5 when a HIgG-HRP is used as detector and TMB as substrate in an ELISA format.
Storage and Stability
The Concanavalin A Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
· Concanavalin A Coated Microplates Specifications
Coating
Concanavalin A is coated using 200 μl/well.
Binding specificity
Biomat has evaluated the binding specificity of its Concanavalin A Coated 96-Well Plate. Click on the following link to see the results.
-
- Properties
- Applications
-
Overview