Concanavalin A Coated - 96 well plates - 8 Well Breakable Strips on single well holding frame

Concanavalin A Coated - 96 well plates - 8 Well Breakable Strips on single well holding frame

Catalog Number:
LC01530722BIO
Mfr. No.:
MG07F/MGW07F/MGB07F2-CON A; MG07F/MGW07F/MGB07F2-CON A/W
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      • Overview
        • Concanavalin A Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.

          Concanavalin A Coated Plates are designed for the specific binding of carbohydrate fractions in glycoproteins, enzymes, and cell membranes. Derived from Canavalia ensiformis (jack bean), Concanavalin A is a lectin commonly used for isolating glyco-conjugates and glycoproteins with specific carbohydrate structures. These plates provide a highly sensitive platform for ELISA assays and are ideal for applications involving glycoproteins, glycopeptides, enzyme-antibody conjugates, as well as interactions with polysaccharides, glycolipids, cell membranes, hormones, and hormone receptors. Their versatility makes them a reliable tool for assays focused on carbohydrate-protein interactions.

          Concanavalin A Coated 96 Well Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

          Concanavalin A Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

          Surface:
          The Concanavalin A surface provide a highly sensitive and specific platform for binding the carbohydrate fractions of glycoproteins, enzymes, and cell membranes. The Concanavalin A plates are ideal for applications such as interactions with glycoproteins, glycopeptides, polysaccharides, and enzymatic-antibody conjugates. Additionally, they support binding with cell membranes, hormones, and hormone receptors, making them a versatile tool for a variety of biochemical and cellular assays.

          Key features of Concanavalin A Plates
          · Concanavalin A Surface:
          · Specifically binds glycoproteins, glycopeptides, and enzyme-antibody conjugates
          · Supports interactions with polysaccharides and glycolipids for versatile applications
          · Facilitates binding to cell membranes, hormones, and hormone receptors
          · Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
          · Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
          · Wells design: flat bottom.
          · Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
          · Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
          Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
          Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
          · Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
          · Alphanumeric Coding: For easy and accurate well identification during experiments.
          · Recommended working volume: of 75 to 200 μl
          · Packaging: Each coated plate is packed in a single barrier bag with desiccant
          · Unit: Contains 5 plates
          · Minimum order: 10 plates.
          · Ready to use

          Key Benefits
          · Plates Design:
          The mould design provides optical quality, important to reduce the background signal.
          The rim protects the external face of the bottom from scratches.
          · Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
          · Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
          · Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
          · Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

          Please contact us at for specific academic pricing.

          Background

          Concanavalin A Coated Surface offers a powerful and sensitive instrument for binding in specific way the carbohydrate fraction of glycoproteins, enzymes and cell membranes.
          Concanavalin A Coated Surface shows specific affinity for molecules containing α-D-mannopyranosyl, α-D-glucopyrannosyl and sterically related and binds in specific way the carbohydrate fraction of glycoproteins, enzymes and cell membranes.

          Concanavalin A, belonging to the lectins family, is a Hemagglutinin from the common jack bean Canavalia ensiformis. It is well known that lectins have been used extensively for the isolation of glyco-conjugates and glycoproteins with specific carbohydrate structures.

          The optical properties of polystyrene remain unchanged, allowing the use of the modified surface as a powerful tool for diagnostic assays.

          · Examples of applications for Concanavalin A Coated surface
          interaction with glycoproteins, glycopeptides and enzyme-antibody conjugates
          polysaccharides and glycolipids
          interaction with cellular membranes, hormones and hormone receptors

          · Concanavalin A Coated Surfaces main features
          Biomat Concanavalin A Coated products have the following characteristics:

          Ready to Use
          Manufactured under ISO:9001 guidelines
          All lots are tested for uniformity and reproducibility
          Certificate of Quality is released for every lot
          For Research Use Only

          · Also, Concanavalin A Coated Products ensures:

          Uniformity
          The Concanavalin A Coated Products show a CV% less than 5 when a HIgG-HRP is used as detector and TMB as substrate in an ELISA format.

          Storage and Stability
          The Concanavalin A Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

          · Concanavalin A Coated Microplates Specifications
          Coating
          Concanavalin A is coated using 200 μl/well.

          Binding specificity
          Biomat has evaluated the binding specificity of its Concanavalin A Coated 96-Well Plate. Click on the following link to see the results.

      • Properties
        • Other Properties
          Material: Polystyrene
          Format: 8 Well Breakable Strip
          Well Volume: 350 µl
          Bottom: Flat
          Color: Black, Clear, White
          Surface: Concanavalin A
          Active surface: 200 µl
          Blocking: Non proteic, Standard
          Uniformity: CV < 5 %
          Shelf life: 2 years
          Packaging: Qty/bag: 1
          Minimum order: 10 pcs
          Unit: 5 pcs
          Compatibility: Compatible with most common automation systems
          Other information: All lots are tested for uniformity and reproducibility Certificate of Quality is released for every lot
          Dimensions: SBS standard
          Storage
          2 – 8°C

          * For research use only.

      • Applications
        • Application Description
          · ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
          · Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
          · Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
          · Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
          · Other

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