Rodent and Human 4MID® Kit (proAKAP4 marker)

The proAKAP4 ELISA Kit - Rodent and human proAKAP4 contains all reagents and buffers required for the quantification of proAKAP4 protein in rodent and human sperm samples (research and preclinical studies).

This ELISA Kit is the 1st testing kit to evaluate sperm quality and spermatozoa functionality in vitro.

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient over time (Sergeant et al. 2019).

High concentrations of #proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in semen samples.

Please contact us at for specific academic pricing.

Background

ProAKAP4 (A–kinase anchor protein 4) has been described over the last decade as a functional marker of spermatozoa. This key protein is largely conserved between species and its specific regulation during spermatogenesis seems essential to increase success rate in artificial insemination settings. Recent cross species analyses have identified the potential use of proAKAP4 as a pertinent biomarker of overall semen quality in clinical settings. In terms of structure, proAKAP4 is a precursor polypeptide that must be converted by motile and alive spermatozoa in mature AKAP4 that consequently will dock and coordinate the core transduction signals regulating sperm functionality, i.e motility, capacitating and fertility.

1. Luconi M, Cantini G, Baldi E, et al. Role of A-kinase anchoring proteins (AKAPs) in reproduction. Front Biosci. 2011;16:1315–1330.
2. Sergeant N, Briand-Amirat L, Bencharif D, et al. The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy and Veterinary Science. 2019;11:01‒19.
3. Delehedde M, Blommaert D, Jouy N, et al. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Rep Sci. 2018;194:224.
4. Delehedde M, Demouveaux B, Remy G, Selleslagh M et al. ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. 2019;7(1):86.

More Details