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Presto™ FFPE RNA Mini Kit with DNase

Presto™ FFPE RNA Mini Kit with DNase

Catalog Number:

NAPK388953GEN

Mfr. No.:

RFD004; RFD050; RFD100

Price:

$110

Size:

Quantity:

Add to Cart:

- Overview
  - The Presto™ FFPE RNA Mini Kit was designed specifically for purifying total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue. Paraffin is initially dissolved with organic solvents such as Xylene followed by Proteinase K digestion. RNA in chaotropic salt is then bound by the glass fiber matrix of the spin column and DNA contamination is removed using an in column DNase treatment. Other contaminants are removed using Wash Buffer and the purified total RNA is eluted in RNase-free Water. High quality total RNA can be purified within 80 minutes without phenol extraction or alcohol precipitation. The purified RNA is ready for use in Real-time PCR, mRNA selection, cDNA synthesis and NGS.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Sample: up to 25 mg sections of FFPE, 4~10 sections of 5~20 μm thick FFPE
    High Yield: up to 50 μg of total RNA
    Spin Columns: glass fiber membrane optimized for total RNA extraction
    Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
    
    Storage
    
    dry at room temperature (15-25°C), DNase I is shipped at room temperature and should be stored at -20°C
    
    * For Research Use Only
- Applications
  - Application
    
    Total RNA Extraction
    
    Application Description
    
    Real-time PCR, mRNA Selection, cDNA Synthesis, NGS

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