pOnebyOne™ V – Neo Bicistronic Mammalian Expression Kit

pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ V (with Neomycin resistance) incorporates the cytomegalovirus early promoter that precedes 2A sequence in frame with the luciferase from the firefly Photinus pyralis. Luciferase is a sensitive enzymatic reporter that can be assayed by standard luciferase activity reaction.

Advantages & Features

Complete solution: a directional cloning vector to clone and produce a protein of interest.

Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter).

Highly efficient: cloning system tested with up to 4 kb inserts.

Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment.

Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest.

Cost avoidance: avoids the use of restriction enzymes.

Accurate: proven performance for most common cell lines.

Flexible: allows transfection in difficult cell lines.

Really low experimental background: lower than 1%.

Convenient: available with different resistance marker cassettes.

Directional cloning: of PCR with the gene of interest.

Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.

Risk-free: product covered by our Quality 100% Guarantee.

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