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Overview
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pOnebyOne™ Multicistronic Vector (EGFP-2A-2A) is an extremely efficient, accurate and flexible Mammalian Expression vector that allows co-expression of two Genes of Interest along with Green Fluorescent Protein (GFP) as reporter gene, driven by Human Cytomegalovirus early promoter. It is based in 2A sequence from Thosea asigna Virus (TaV) which is flanked by two Multiple Cloning sites. Stoichiometric production of Proteins. Stable Mammalian Cells could be selected by Neomycin resistance. Other Resistance or Markers may be offered upon request.
Advantages & Features
Complete solution: a cloning vector to clone and produce two proteins of interest, plus a reporter.
Breakthrough technology: based in 2A sequence simultaneous expression of 3 proteins in mammalian cells.
Highly efficient: tested with up to 4 kb inserts.
Accurate: proven performance for most common cell lines.
Flexible: allows transfection in difficult cell lines.
Convenient: available with different resistance marker cassettes.
Reporter checking: stoichiometric amount of your proteins of interest and a reporter protein.
Risk-free: product covered by our Quality 100% Guarantee.Please contact us at for specific academic pricing.
More Details
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- Properties
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Overview