pOnebyOne™ I – Neo Bicistronic Mammalian Expression Kit

pOnebyOne™ I – Neo Bicistronic Mammalian Expression Kit

Catalog Number:
ME01108678CAN
Mfr. No.:
ME001-N-S; ME001-N
Price:
$477
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      • Overview
        • pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ I (with Neomycin resistance) incorporates the cytomegalovirus early promoter that precedes 2A sequence in frame with truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones. It could be visualized by cytometry using specific antibody labelled with FITC or similar and also, it could be enriched from negative clones with magnetic beads bearing anti-ΔNGFR antibody.

          Advantages & Features

          Complete solution: a directional cloning vector to clone and produce a protein of interest.

          Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter).

          Highly efficient: cloning system tested with up to 4 kb inserts.

          Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment.

          Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest.

          Cost avoidance: avoids the use of restriction enzymes.

          Accurate: proven performance for most common cell lines.

          Flexible: allows transfection in difficult cell lines.

          Really low experimental background: lower than 1%.

          Convenient: available with different resistance marker cassettes.

          Directional cloning: of PCR with the gene of interest.

          Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.

          Risk-free: product covered by our Quality 100% Guarantee.

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Categories
          Mammalian Expression
          Storage
          -20 °C (NON Frost-Free Freezer). 24 months from the date of purchase, if it is properly stored.
          Shipping
          Gel pack.

          * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.

      • Applications
        • Application Description
          Protein Expression of intracellular, extracellular or transmembrane higher cells in an equimolecular to a surface marker that allows quantification.

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