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Poly(A) Polymerase, Yeast

Poly(A) Polymerase, Yeast

Catalog Number:

E001278646APP

Mfr. No.:

APPL_E017

Price:

$205

Size:

100 U (100 μl)

Quantity:

Add to Cart:

- Overview
  - Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
    
    Please contact us at for specific academic pricing.
- Properties
  - Type
    
    DNA and RNA Mesophilic Polymerases
    
    Formulation
    
    20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
    
    Storage
    
    Store all components at -20°C.
    
    Concentration
    
    1 U/μl
    
    * For research use only. Not for diagnostic or therapeutic use.
- Applications
  - Application Description
    
    • Labelling of RNA with ATP or cordycepin
    • Poly(A) tailing of RNA for cloning or affinity purification
    • Increasing translation of RNA transferred into eukaryotic cells

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