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Ultra-Pure Phi29 DNA Polymerase

Ultra-Pure Phi29 DNA Polymerase

Catalog Number:

E001278647APP

Mfr. No.:

APPL_E014

Price:

$364

Size:

1000 U (100 μl)

Quantity:

Add to Cart:

- Overview
  - Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates.
    
    Please contact us at for specific academic pricing.
- Properties
  - Type
    
    DNA and RNA Mesophilic Polymerases
    
    Formulation
    
    50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and 50% (v/v) Glycerol.
    
    Storage
    
    Store all components at -20°C.
    
    Concentration
    
    10 U/μl
    
    * For research use only. Not for diagnostic or therapeutic use.
- Applications
  - Application Description
    
    • Rolling circle amplification (RCA)
    • Multiple displacement amplification (MDA)
    • Whole genome amplification (WGA)
    • DNA template preparation for sequencing
    • Protein-primed DNA amplification

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